Cytotoxicity of newly developed pozzolan cement and other root-end filling materials on human periodontal ligament cell
송민주, 윤태선, 김수연, 김의성,
소속 상세정보
송민주 ( Song Min-Ju ) - Yonsei University College of Dentistry Gangnam Severance Dental Hospital Department of Conservative Dentistry
윤태선 ( Yoon Tae-Sun ) - Yonsei University College of Dentistry Department of Conservative Dentistry
김수연 ( Kim Sue-Youn ) - Yonsei University College of Dentistry Department of Conservative Dentistry
김의성 ( Kim Eui-Seong ) - Yonsei University College of Dentistry Department of Conservative Dentistry and Oral Science Research Center
KMID : 1034420140390010039
Abstract
Objectives; The purpose of this study was to evaluate in vitro cytotoxicity of the pozzolan cement and other root-end filling materials using human periodontal ligament cell.
Materials and Methods: Endocem (Maruchi), white ProRoot MTA (Dentsply), white Angelus MTA (Angelus), and Super EBA (Bosworth Co.) were tested after set completely in an incubator at 37℃ for 7 days, Endocem was tested in two ways: 1) immediately after mixing (fresh specimens) and 2) after setting completely like other experimental materials. The methods for assessment included light microscopic examination, cell counting and WST-1 assay on human periodontal ligament cell.
Results: In the results of microscopic examination and cell counting, Super EBA showed significantly lower viable cell than any other groups (p < 0.05). As the results of WST-1 assay, compared with untreated control group, there was no significant cell viability of the Endocem group. However, the fresh mixed Endocem group had significantly less cell viability. The cells exposed to ProRoot MTA and Angelus MTA showed the highest viability, whereas the cells exposed to Super EBA displayed the lowest viability (p < 0.05).
Conclusions: The cytotoxicity of the pozzolan cement (Endocem) was comparable with ProRoot MTA and Angelus MTA. Considering the difficult manipulation and long setting time of ProRoot MTA and Angelus MTA, Endocem can be used as the alternative of retrofilling material.
키워드
Cytotoxicity; Endocem; Pozzolan cement; Root-end filling material; WST-1 assay
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